ABSTRACT The project ‘One Health in the Pantanal: society’s participation in the emergency surveillance of zoonoses emergency as a post-fire effect in the territory and the formation of integrated strategies’ aimed to integrate institutional representations and local society; expand the use of Wildlife Health Information System (SISS-Geo) for fauna monitoring; identify priority areas for zoonosis surveillance and build paths involving One Health (OH). A webinar was held, highlighting the need for broader events with the participation of leaders in each of the chosen territories. Seminars and workshops were held in the states of Mato Grosso do Sul (MS), with the participation of health service managers from the Province of Santa Cruz, Bolivia, and Mato Grosso. The representation of different segments at the events enabled the articulation of citizens and local managers. In traditional communities, it was possible to address the impacts of the fires and provide the opportunity for these people to express their health priorities and demands, before and after the fires. The Synthesis Workshop held in Corumbá, MS allowed the feedback of results and integration with representatives from different institutions in Brazil and Bolivia, and prospecting and prioritization of illnesses to be incorporated into the OH model for the Pantanal and western border of Brazil.

RESUMO O projeto ‘Saúde Única no Pantanal: participação da sociedade na vigilância de emergência de zoonoses como efeito pós-incêndios no território e formação de estratégias integradas’ objetivou integrar representações institucionais e da sociedade local; ampliar o uso do Sistema de Informação em Saúde Silvestre (SISS-Geo) para o monitoramento da fauna; identificar áreas prioritárias para vigilância de zoonoses e construir caminhos envolvendo a Saúde Única (SU). Realizou webinário, apontando a necessidade de eventos mais amplos com a participação de lideranças em cada um dos territórios escolhidos. Foram executados seminários e oficinas nos estados de Mato Grosso do Sul (MS), com a participação de gestores do serviço de saúde da Província de Santa Cruz, Bolívia, e de Mato Grosso. A representatividade dos diferentes segmentos nos eventos possibilitou a articulação de cidadãos e gestores locais. Nas comunidades tradicionais, foi possível abordar os impactos dos incêndios e dar oportunidade para que essas pessoas manifestassem suas prioridades e demandas de saúde, antes e depois dos incêndios. A Oficina Síntese realizada em Corumbá, MS possibilitou a devolutiva dos resultados e a integração com representantes de diferentes instituições do Brasil e da Bolívia, além da prospecção e priorização de enfermidades a serem incorporadas em modelo de SU para o Pantanal e fronteira oeste do Brasil.

BACKGROUND Few studies have focused on microbial diversity in indoor environments of ships, as well as the role of the microbiome and its ecological interconnections. In this study, we investigated the microbiome and virome present on the internal surfaces of a polar ship in different stages (beginning, during, and at the end) of the Brazilian Antarctic expedition in order to evaluate abundance of microorganisms in different periods. OBJECTIVES AND METHODS We used shotgun metagenomic analysis on pooled samples from sampling surfaces in the ship’s interior to track the microbial diversity. FINDINGS Considering the total fraction of the microbiome, the relative abundance of bacteria, eukaryotes, viruses, and archaea was 83.7%, 16.2%, 0.04%, and 0.002%, respectively. Proteobacteria was the most abundant bacterial phyla, followed by Firmicutes, Actinobacteria, and Bacteroidetes. Concerning the virome, the greatest richness of viral species was identified during the middle of the trip, including ten viral families after de novo assembly: Autographiviridae, Chrysoviridae, Genomoviridae, Herelleviridae, Myoviridae, Partitiviridae, Podoviridae, Potyviridae, Siphoviridae, and Virgaviridae. MAIN CONCLUSIONS This study contributed to the knowledge of microbial diversity in naval transportation facilities, and variations in the abundance of microorganisms probably occurred due to factors such as the number of passengers and activities on the ship.

ABSTRACT. Two muriqui species are currently described in the literature: the southern muriqui, Brachyteles arachnoides (E. Geofroy, 1806) and the northern muriqui, Brachyteles hypoxanthus (Kuhl, 1820). Nowadays, the distribution of the southern muriqui is restricted to forest patches of the states of Rio de Janeiro, São Paulo and Paraná, while the northern muriqui occurs in Espírito Santo, Bahia and Minas Gerais. Both are threatened species, but there are still many gaps in our knowledge of their occurrence, especially for southern muriquis in the state of RJ. The purpose of this work is precisely to identify the primary areas of occurrence of the muriqui in Rio de Janeiro through historical reports by naturalists, adventurers and others since the 16th century. Thus, allowing to assist in the establishment and conservation strategies of the species.

RESUMO. Existem duas espécies de muriquis descritas atualmente na literatura: muriqui do sul, Brachyteles arachnoides (E. Geoffroy, 1806) e muriqui-do-norte, Brachyteles hypoxanthus (Kuhl, 1820). Atualmente, o muriqui-do-sul tem sua distribuição restrita a fragmentos florestais dos estados do Rio de Janeiro, São Paulo e Paraná, enquanto o muriqui do norte ocorre no Espírito Santo, Bahia e Minas Gerais. Ambas são espécies ameaçadas, mas ainda existem muitas lacunas no conhecimento de sua ocorrência, principalmente para os muriquis-do-sul do estado do RJ. O intuito desse trabalho é justamente identificar as áreas primárias da ocorrência do muriqui no Rio de Janeiro por meio de relatos históricos de naturalistas, aventureiros e outros desde o século XVI, permitindo assim, auxiliar no estabelecimento e estratégias de conservação da espécie.

BACKGROUND Herpesvirus transmission between humans and non-human primate (NHP) can occur through contact scratches with lesions, infected saliva, and mainly through contaminated food. Therefore, cross-infection can lead to severe illness or even death for both the animal and human. In 2017, during the yellow fever (YF) outbreak in Brazil, species of the New World Primates (NWP) from Rio de Janeiro state, tested negative for yellow fever virus (YFV) detection. OBJECTIVES To evaluate herpesvirus in the population NWP in Rio de Janeiro. METHODS To investigate, liver samples of 283 NWP, from several regions of the state of Rio de Janeiro, were tested for the herpesvirus family using a Pan-polymerase chain reaction (Pan-PCR) and sequencing. FINDINGS 34.6% (98/283) tested positive for at least one herpesvirus; 29.3% (83/283) tested positive to Human alphaherpesvirus 1 (HSV-1), this virus from humans can be lethal to New World monkey; 13% (37/283) were detected Callitrichine gammaherpesvirus 3 (CalHV-3), responsible for lymphoproliferative disease that can be fatal in NWP. In addition, CalHV-3 / HSV-1 co-infection was in 11.6% (33/283) of the samples. MAIN CONCLUSIONS Pan-herpesvirus was useful to identify species-specific herpesviruses and virus from human that can infect animals. Furthermore, during an outbreak of YF other infections should be monitored.

The 2030 Agenda for Sustainable Development is a plan of action for people, planet and prosperity. Thousands of years and centuries of colonisation have passed the precarious housing conditions, food insecurity, lack of sanitation, the limitation of surveillance, health care programs and climate change. Chagas disease continues to be a public health problem. The control programs have been successful in many countries in reducing transmission by T. cruzi; but the results have been variable. WHO makes recommendations for prevention and control with the aim of eliminating Chagas disease as a public health problem. Climate change, deforestation, migration, urbanisation, sylvatic vectors and oral transmission require integrating the economic, social, and environmental dimensions of sustainable development, as well as the links within and between objectives and sectors. While the environment scenarios change around the world, native vector species pose a significant public health threat. The man-made atmosphere change is related to the increase of triatomines’ dispersal range, or an increase of the mobility of the vectors from their sylvatic environment to man-made constructions, or humans getting into sylvatic scenarios, leading to an increase of Chagas disease infection. Innovations with the communities and collaborations among municipalities, International cooperation agencies, local governmental agencies, academic partners, developmental agencies, or environmental institutions may present promising solutions, but sustained partnerships, long-term commitment, and strong regional leadership are required. A new world has just opened up for the renewal of surveillance practices, but the lessons learned in the past should be the basis for solutions in the future.

We present the results of paleoparasitological analyses in coprolites of Kerodon rupestris, rodent endemic to rocky areas of Brazil's semiarid region. The coprolites were collected from excavations at the archaeological site of Toca dos Coqueiros, in the National Park of Serra da Capivara, southeastern of state of Piauí. Syphacia sp. (Nematoda: Oxyuridae) eggs were identified in coprolites dated at 5,300 ± 50 years before present. This is the first record of the genus Syphacia in rodent coprolites in the Americas.

We present results of the paleoparasitological analysis of Cervidae coprolites that were recovered from the archaeological site Furna do Estrago, Pernambuco, Brazil. Trichuris sp. eggs were recovered from the coprolite samples dated 1,040 ± 50 years before present. This is the first record of Trichuris sp. in semiarid Cervidae, unexpectedly recorded in archaeological material.

The aim of this work was to study the degradation and detoxification of three textile azo dyes (Reactive Red 198, Reactive Red 141 and Reactive Blue 214) by mixed fungal cultures from semi-arid region of Brazilian Northeast. Sediment samples of twenty water reservoirs in the surroundings of Serra da Capivara National Park, area of environmental preservation in the caatinga in the State of Piauí, with semi-arid climate, were evaluated in order to select the consortia of fungi capable to degrade and detoxify these dyes. The mixed fungal culture from Caldeirão Escuridão (CE) reservoir was the most efficient in the degradation and detoxification of the dyes tested.

Estudos sobre biodiversidade permitem avaliações ecossistêmicas e monitoramentos de impactos e mudanças ambientais. A diversidade parasitária pode refletir o processo coevolutivo entre parasitos e hospedeiros, e mudanças ambientais que permitem a perda, ganho ou a manutenção das espécies nesse processo. O presente trabalho utilizou espécies/morfotipos de ovos de helmintos encontrados em fezes de sete mamíferos silvestres (os grupos Dasypodidae e grandes felinos, e as espécies: Tamandua tetradactyla, Cebus apella, Alouatta caraya, Cerdocyon thous, Pecari tajacu) e dois domésticos (Canis familiaris e Sus scrofa), que cohabitam o Parque Nacional Serra da Capivara (PNSC) e seu entorno, para analisar a diversidade dos helmintos intestinais desses mamíferos. Neste trabalho, utilizou-se achados da fauna helmintológica de mamíferos silvestres e domésticos, para avaliar um possível fluxo de helmintos entre estes dois grupos hospedeiros usando Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dos hospedeiros com base na composição de suas faunas helmintológicas. Os resultados indicam que a região do PNSC, ainda mantém condições ambientais que ainda propiciam a manutenção da composição da fauna de helmintos de mamíferos silvestres, diferente da encontrada nos animais domésticos.

Biodiversity studies allow ecosystem assessment and monitoring of environmental changes and impacts. Parasite diversity could reflect the host/ parasite coevolutionary process and the environment changes that permit the loss, gain or maintenance of species. This survey used species/morphotypes of helminths eggs found in feces from seven wild mammal species (the groups Dasypodidae and Large Cats, and Tamandua tetradactyla, Cebus apella, Alouatta caraya, Cerdocyon thous, Pecari tajacu) and from two domestic species (Canis familiaris and Sus scrofa), which occur within the Serra da Capivara National Park (PNSC) and surrounding areas in order to analise the diversity of mammal intestinal helminths. This work used the helminthological fauna findings of wild and domestic mammals, to consider a possible helminth flux between these two host groups using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) of the hosts based on helminthological fauna composition. The results indicate that the region of the PNSC still maintains environmental conditions that still keep wild mammal helminthological fauna composition different from the one found for domestic mammals.

São revistos os registros de ocorrência de helmintos intestinais parasitos de animais em vestígios arqueológicos humanos, relatados desde o surgimento dos estudos paleopatológicos. Busca-se relacionar os achados em paleoparasitologia com fatores geográficos, bióticos e abióticos do ambiente em que as populações pré-históricas viviam, e com aspectos do processo de dispersão e evolução biológica e cultural humana. A modificação de hábitos alimentares e a incorporação de novas práticas culturais são analisadas sob o ponto de vista das zoonoses desde a pré-história até a atualidade, em especial em populações indígenas brasileiras. Três tabelas identificando os helmintos, seus hospedeiros naturais, datações e local dos achados arqueológicos complementam esta revisão. Conclui-se que várias zoonoses conhecidas hoje ocorrem desde a antiguidade e que estes dados, combinados a estudos de emergência e reemergência de doenças, podem auxiliar a compor cenários para o futuro.

The authors present a review of records of intestinal parasitic helminths from animals in human archaeological remains, reported since the emergence of paleopathological studies. The objective was to relate paleoparasitological findings to geographic, biotic, and abiotic factors from the environment in which the prehistoric populations lived, and understand some aspects related to the process of human dispersion and biological and cultural evolution. Modification of eating habits and the incorporation of new cultural practices are analyzed from the perspective of zoonoses from prehistory to the present day, especially in Brazilian indigenous populations. Three tables identifying the helminths, their natural hosts, dates, and sites of archaeological findings complete this review. In conclusion, various zoonoses known today have occurred since antiquity, and these data, combined with studies on the emergence and reemergence of diseases, could make possible to compose scenarios for the future.

The study of feces of terrestrial mammals brings out biological and ecological data such as the species presence, diet, behaviour, territory, parasitic fauna, and home-range use, which can be applied for conservation projects and support paleoecological research that use coprolites as the main source of study. Although the new biotechnological techniques allow more accurate data, the diagnosis based on morphometric analyses permits the primary identification of the taxonomic group origin to support the best choice of subsequent analyses. We present the compilation list of fecal shape and measurements available in the literature published in North America, Eastern and Southern Africa, Europe, and new data from Brazil. Shape and diameters are the best characteristics for taxonomic identification. Feces were assembled in 9 groups that reflect the Order, sometimes the Family, and even their common origin.

Ovos e larvas de Strongyloides ferreirai Rodrigues, Vicente & Gomes, 1985 foram identificados em coprólitos de Kerodon rupestris (Wied.), datados de 8.000 a 2.000 anos AP, coletados em sítios arqueológicos do nordeste do Brasil.

Eggs and larvae of Strongyloides ferreirai Rodrigues, Vicente & Gomes, 1985 are identified in Kerodon rupestris (Wied.) coprolites dated from 8.000-2.000 years BP (Before Present), collected from archaeological sites from the northeast of Brazil.

Uma questão central em paleoparasitologia é o diagnóstico correto da origem dos coprólitos encontrados em sítios arqueológicos. A separação entre coprólitos humanos e animais, através do estudo do tamanho, forma, características após a reidratação, conteúdo alimentar e a presença de parasitos, tem sido usada principalmente nos casos de material de origem humana. Em relação aos coprólitos de animais, um método que provou ser eficiente é a comparação de coprólitos com fezes recentes de animais da região dos sítios arqueológicos, seguindo-se os parâmetros mencionados anteriormente. Este trabalho refere-se ao diagnóstico de coprólitos de tamanduá (Tamandua tetradactyla; Mymercophaga tridactyla) com a presença de ovos de Gigantorynchus echinodiscus (Archiancanthocephala; Gigantorynchidae).

An important point in paleoparasitology is the correct diagnosis of the origin of coprolites found in archaelogical sites. The identification of human and animal coprolites, through the study of the shape, size, charactheristics after rehydration, alimentary contents, and the presence of parasites, has proved to be accurate for human coprolites. For non-human ones we compared coprolites with recent faeces of animals collected near the archaeological sites, following the methodology above mentioned. In this paper anteaters coprolites (Tamandua tetradactyla; Mymecophaga tridactyla) with eggs of Gigantorhynchus echinodiscus (Archiancanthocephala; Gigantorynchidae) were identified.

A ocorrência de certos parasitos em grupos humanos de diferentes regiões tem sido usada para o estudo da origem geográfica tanto da população humana como de seus parasitos. A presença da ancilostomose, em tribos isoladas de regiões remotas do Paraguai, levou a especulações sobre a sua origem, uma vez que as migrações pré-históricas pelo caminho de Bering não poderiam ter sido responsáveis pela introdução do parasito que necessita, para sua manutenção no solo, de temperatura em torno de 22ºC. Propôs-se então que somente migrações transpacíficas de populações asiáticas poderiam ter intoduzido o parasito na América. Esta discussão data do início do século e somente com os recentes achados da paleoparasitologia foi retomada novamente. Este trabalho é uma revisão sobre o encontro de ancilostomídeos em material arqueológico e sugere possíveis rotas seguidas pelos hospedeiros para alcançar o Novo Mundo.

The occurrence of certain parasites in human groups from different regions has been used to study the geographical origin of both hosts and parasites. The presence of hookworm infection in an isolated tribe in remote regions of Paraguay led to speculations about its origin, since the prehistoric migrations through the Behring Land Bridge could not have brought the parasite, which needs for its mantenance a temperature of about 22ºC in the soil. It was then proposed that only transpacific migrations of Asiatic populations could have brought the parasite to America. This discussion dates back to the beginning of this Century and it was only with recent paleoparasitological findings that it arose again. This paper is a review of hookworm findings in archaeological material and suggests possible routes followed by their hosts to reach the New World.